Effects of foliar biostimulant formulation and dose on fruit development and quality of sweet granadilla (Passiflora ligularis Juss.)
DOI:
https://doi.org/10.63804/saastal.v2i2.e1Keywords:
Andean crops, foliar application, fruit quality, plant biostimulants, total soluble solidsAbstract
Plant biostimulants represent a promising alternative for improving production efficiency and fruit quality; however, limited information is available regarding their application to sweet granadilla (Passiflora ligularis Juss.). This study evaluated the effects of formulation and dose of two foliar biostimulants on sweet granadilla fruit development and quality under Andean conditions. Crop+Plus and Kelan Complex were applied at 0.0, 1.0, 1.5, and 2.0 mL·L⁻¹ in a randomised complete block design with split plots. Ten fruits were selected from each experimental unit, and their polar and equatorial diameters were measured at 0, 24, and 50 days after the first application. Total soluble solids, individual fruit weight, and weight-based estimated yield were determined at harvest. Greater polar and equatorial diameters were observed when Crop+Plus was applied, with significant effects of time and their interaction. At 50 days, Crop+Plus at 2.0 mL·L⁻¹ produced the largest polar and equatorial diameters, reaching 7.70 and 7.43 cm, respectively. Total soluble solids were affected by formulation and dose, whereas fruit weight was mainly influenced by dose. The most favourable results were obtained with Crop+Plus at 2.0 mL·L⁻¹, which produced 20.90 °Brix, a mean fruit weight of 120.0 g, and an estimated yield of 6,720 kg·ha⁻¹. In conclusion, Crop+Plus exerted the most consistent effects on fruit growth and quality, and 2.0 mL·L⁻¹ was the most promising concentration within the range evaluated.
References
S. Araya, A. M. Martins, N. T. V Junqueira, A. M. Costa, F. G. Faleiro, and M. E. Ferreira, “Microsatellite marker development by partial sequencing of the sour passion fruit genome (Passiflora edulis Sims),” BMC Genomics, vol. 18, no. 1, p. 549, Dec. 2017, doi: https://doi.org/10.1186/s12864-017-3881-5.
G. Fischer and D. Miranda, “Review on the ecophysiology of important Andean fruits: Passiflora L.,” Rev. Fac. Nac. Agron. Medellin, vol. 74, no. 2, pp. 9471–9481, May 2021, doi: https://doi.org/10.15446/rfnam.v74n2.91828.
J. C. Carrasco, L. F. L. Palaquibay, and V. Caballero-Serrano, “Fruit characterization of Passiflora ligularis in different Andean regions of Ecuador,” Horticulturae, vol. 11, no. 9, p. 1058, Sep. 2025, doi: https://doi.org/10.3390/horticulturae11091058.
F. J. Muñoz-Ordoñez, N. Gutiérrez-Guzmán, M. S. Hernández-Gómez, and J. P. Fernández-Trujillo, “The climactic conditions limit fruit production and quality in gulupa (Passiflora edulis Sims f. edulis) under integrated fertilization,” South African Journal of Botany, vol. 153, pp. 147–156, Feb. 2023, doi: https://doi.org/10.1016/j.sajb.2022.11.043.
P. Gaona-Gonzaga et al., “Response of sweet passion fruit (Passiflora liguralis Juss) cultivar ‘Colombiana’ to the supply of nitrogen and potassium through fertirrigation,” Manglar, vol. 17, no. 1, pp. 75–82, Jun. 2020, doi: https://doi.org/10.17268/manglar.2020.012.
G. S. Mattar, C. C. de Moraes, L. M. M. Meletti, and L. F. V. Purquerio, “Accumulation and exportation of nutrients by yellow Passion fruit cv. IAC 275,” Rev. Bras. Frutic., vol. 40, no. 3, p. e178, Jun. 2018, doi: https://doi.org/10.1590/0100-29452018178.
S. Menegat, A. Ledo, and R. Tirado, “Greenhouse gas emissions from global production and use of nitrogen synthetic fertilisers in agriculture,” Sci. Rep., vol. 12, no. 1, p. 14490, Aug. 2022, doi: https://doi.org/10.1038/s41598-022-18773-w.
Y. Wang et al., “Preventing subsoil enhanced nitrification to safeguard agroecosystem sustainability,” Nat. Commun., vol. 17, no. 1, p. 3648, Mar. 2026, doi: https://doi.org/10.1038/s41467-026-70277-7.
P. Quille, J. Kacprzyk, S. O’Connell, and C. K. Y. Ng, “Reducing fertiliser inputs: plant biostimulants as an emerging strategy to improve nutrient use efficiency,” Discover Sustainability, vol. 6, no. 1, p. 128, Feb. 2025, doi: https://doi.org/10.1007/s43621-025-00910-w.
L. Di Sario, P. Boeri, J. T. Matus, and G. A. Pizzio, “Plant biostimulants to enhance abiotic stress resilience in crops,” Int. J. Mol. Sci., vol. 26, no. 3, p. 1129, Jan. 2025, doi: https://doi.org/10.3390/ijms26031129.
European Parliament and Council of the European Union, Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products. , L 170, . Unión Europea: Official Journal of the European Union, 2019, pp. 1–114.
M. Baltazar, S. Correia, K. J. Guinan, N. Sujeeth, R. Bragança, and B. Gonçalves, “Recent advances in the molecular effects of biostimulants in plants: An oerview,” Biomolecules, vol. 11, no. 8, p. 1096, Jul. 2021, doi: https://doi.org/10.3390/biom11081096.
P. du Jardin, “Plant biostimulants: Definition, concept, main categories and regulation,” Sci. Hortic., vol. 196, pp. 3–14, Nov. 2015, doi: https://doi.org/10.1016/j.scienta.2015.09.021.
M. Ruzzi, G. Colla, and Y. Rouphael, “Biostimulants in agriculture II: towards a sustainable future,” Front. Plant Sci., vol. 15, p. 1427283, May 2024, doi: https://doi.org/10.3389/fpls.2024.1427283.
D. Roche, J. R. Rickson, and M. Pawlett, “Moving towards a mechanistic understanding of biostimulant impacts on soil properties and processes: a semi-systematic review,” Frontiers in Agronomy, vol. 6, p. 1271672, May 2024, doi: https://doi.org/10.3389/fagro.2024.1271672.
J. Li, T. Van Gerrewey, and D. Geelen, “A meta-analysis of biostimulant yield rffectiveness in field trials,” Front. Plant Sci., vol. 13, p. 836702, Apr. 2022, doi: https://doi.org/10.3389/fpls.2022.836702.
J. L. Santos-Jiménez et al., “Passion fruit plants treated with biostimulants induce defense-related and phytohormone-associated genes,” Plant Gene, vol. 30, p. 100357, Jun. 2022, doi: https://doi.org/10.1016/j.plgene.2022.100357.
A. M. Orjuela-Rodríguez, J. E. Castilla-Baquero, and H. E. Balaguera-López, “Can biostimulants and grafting alleviate salinity stress in purple passion fruit (Passiflora edulis f. edulis Sims)?,” Agron. Colomb., vol. 42, no. 1, p. e113585, Apr. 2024, doi: https://doi.org/10.15446/agron.colomb.v42n1.113585.
L. P. Canellas, N. A. Canellas, D. Martinez-Balmori, R. M. Silva, R. C. C. Rosa, and F. L. Olivares, “Farm-produced plant biostimulant: Case study with passion fruit,” Agronomy, vol. 15, no. 3, p. 681, Mar. 2025, doi: https://doi.org/10.3390/agronomy15030681.
W. Sun, M. H. Shahrajabian, Y. Kuang, and N. Wang, “Amino acids biostimulants and protein hydrolysates in agricultural sciences,” Plants, vol. 13, no. 2, p. 210, Jan. 2024, doi: https://doi.org/10.3390/plants13020210.
M. Malécange, R. Sergheraert, B. Teulat, E. Mounier, J. Lothier, and S. Sakr, “Biostimulant properties of protein hydrolysates: Recent advances and future challenges,” Int. J. Mol. Sci., vol. 24, no. 11, p. 9714, Jun. 2023, doi: https://doi.org/10.3390/ijms24119714.
A. Agosti et al., “Closing the loop in agriculture: Evaluation of the effects of fermented tomato-based biostimulant on Solanum lycopersicum (L.) growth and fruit quality,” Plant Science, vol. 363, p. 112865, Feb. 2026, doi: https://doi.org/10.1016/j.plantsci.2025.112865.
V. S. Rana et al., “Seaweed extract as a biostimulant agent to enhance the fruit growth, yield, and quality of kiwifruit,” Horticulturae, vol. 9, no. 4, p. 432, Mar. 2023, doi: https://doi.org/10.3390/horticulturae9040432.
B. Gonçalves et al., “Biostimulants in fruit crop production: Impacts on growth, yield, and fruit quality,” Horticulturae, vol. 11, no. 12, p. 1452, Dec. 2025, doi: https://doi.org/10.3390/horticulturae11121452.
H. Y. Dasgan, K. S. Aksu, K. Zikaria, and N. S. Gruda, “Biostimulants enhance the nutritional quality of soilless greenhouse tomatoes,” Plants, vol. 13, no. 18, p. 2587, Sep. 2024, doi: https://doi.org/10.3390/plants13182587.
A. Cirillo et al., “Optimizing apricot yield and quality with biostimulant interventions: A comprehensive analysis,” Horticulturae, vol. 10, no. 5, p. 447, Apr. 2024, doi: https://doi.org/10.3390/horticulturae10050447.
L. Vultaggio et al., “Joint action of Trichoderma atroviride and a vegetal derived-protein hydrolysate improves performances of woodland strawberry in Italy,” Horticulturae, vol. 10, no. 5, p. 459, Apr. 2024, doi: https://doi.org/10.3390/horticulturae10050459.
H. Irani, B. ValizadehKaji, and M. R. Naeini, “Biostimulant-induced drought tolerance in grapevine is associated with physiological and biochemical changes,” Chemical and Biological Technologies in Agriculture, vol. 8, no. 1, p. 5, Dec. 2021, doi: https://doi.org/10.1186/s40538-020-00200-9.
A. El Chami et al., “Protein hydrolysates modulate quality traits of tomato fruit under salt stress by regulating the expression patterns of genes related to sugar metabolism,” Horticulturae, vol. 11, no. 9, p. 1108, Sep. 2025, doi: https://doi.org/10.3390/horticulturae11091108.
P. Zydlik, Z. Zydlik, and N. E. Kafkas, “The effect of the foliar application of biostimulants in a strawberry field plantation on the yield and quality of fruit, and on the content of health-beneficial substances,” Agronomy, vol. 14, no. 8, p. 1786, Aug. 2024, doi: https://doi.org/10.3390/agronomy14081786.
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